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Subcutaneous DPSC transplants in immunocompromised mice (A-C) and characterization of DPSC-mediated dentinogenesis in vivo (D-F). (A) Four wks after transplantation, DPSCs differentiated into odontoblasts (open arrows) responsible for new dentin (D) formation on the surface of the HA/TCP (HA). (B,C) At 8 and 16 wks post-transplantation, respectively. (D) Newly formed reparative dentin-like structure (ND) attached to the surfaces of human dentin in DPSC/dentin transplants. BV, blood vessels; CT, connective tissue; dentinogenic cells (black arrowheads). DPSCs formed reparative dentin-like structure containing entrapped cells (open arrowheads). (E) In DPSC/dentin transplants, dentinogenic cells (open arrows) and trapped cells (open arrowheads) within the newly formed reparative dentin-like structure (ND) were immunoreactive to human DSP antibody, as was the pre-existing dentin (black arrows). (F) Staining of human-specific anti-mitochondria antibody, showing the human origin of DPSCs (open arrows). Bar, 40 µm in A-C, 20 µm in D-F (adapted from Batouli et al., 2003). 153554b96e
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